Clinical evaluation of a new polymerase chain reaction assay for detection of Chlamydia trachomatis in endocervical specimens
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منابع مشابه
Clinical evaluation of a new polymerase chain reaction assay for detection of Chlamydia trachomatis in endocervical specimens.
A clinical evaluation of the Amplicor polymerase chain reaction (PCR) assay for the detection of Chlamydia trachomatis in endocervical swabs (Roche Molecular Systems, Branchburg, N.J.) is described. This new clinical system used one-step sample preparation, amplification with biotinylated cryptic plasmid primer pairs (CP24-CP27), uracil-N-glycosylase (AmpErase), and a microtiter format for ampl...
متن کاملCOMPARISON OF CULTURE WITH POLYMERASE CHAIN REACTION FOR DETECTION OF UREAPLASMA UREALYTICUM IN ENDOCERVICAL SPECIMENS
Background: Ureaplasma urealyticum is known as a sexually transmitted agent, causing mainly urethritis, pelvic int1ammatory disease, spontaneous abortion, pyelonephritis, infertility, stillbirth, low birth weight neonatal meningititis, and neonatal pneumonia. U. urealyticum infections not only jeopardize fertility but also pose a risk for infertility treatment and resulting pregnancies. Dia...
متن کاملReproducibility of the Roche Amplicor polymerase chain reaction assay for detection of infection by Chlamydia trachomatis in endocervical specimens.
tion of traditional, iron-laden beer, and two-fifths of them had increased serum ferritin. In contrast to our studies, Wapnick et al. (7) reported that serum iron concentrations increased dramatically after oral administration of vitamin C to iron-loaded, scorbutic subjects. The explanations for this difference probably lie in the timing of the vitamin C dose in relation to the serum iron measu...
متن کاملDetection of Chlamydia trachomatis endocervical infections by ligase chain reaction versus ACCESS Chlamydia antigen assay.
Ligase chain reaction (LCR) was compared with ACCESS immunoassay for detection of chlamydial infections in females. Despite efforts to improve ACCESS performance by evaluation of specimens that were in the test performance "grey zone," LCR remained more sensitive and was less expensive to perform. ACCESS had a sensitivity of 83.9%, a specificity of 99.7%, a positive predictive value of 96.3%, a...
متن کاملGenotyping of Chlamydia trachomatis from endocervical specimens in Shiraz, Iran
The objective of this study was to determine the prevalence and distribution of Chlamydia trachomatisgenotypes in Shiraz, Iran. Two hundred twelve cervical swab samples were collected from women attendingShahid Motahari Polyclinic in Shiraz, Iran. The endocervical specimens were screened for C. trachomatis byplasmid PCR. Genotyping was performed in C. trachomatis-positive samples by nested PCR ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1993
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.31.10.2648-2653.1993